Laboratory of Bioorganic Chemistry of
Natural Products
Previous name: Laboratory of
Biophysical Chemistry
Graduate School of Agricultural
Science, Tohoku University
468-1 Aramaki-Aza-Aoba, Aoba-ku, Sendai
980-8572 Japan
(10th April 2025)
Welcome to our laboratory!
We started our laboratory April, 2004
with new group members. Our major interest is in the chemistry and pharmacology
of natural products.
Especially, we are studying on natural
toxins from the marine animals and organisms.
Current research projects:
1. Tetrodotoxin (puffer fish toxin):
Origin, Biosynthesis, Natural analogues, Analytical methods
(LC/MS), Binding model to Na+
channel, Binding protein in puffer fish, Resistant systems against toxins, Metabolism.
2. Okadaic acid : Binding proteins from
the sponge Halichondria okadai
3. Paralytic shellfish poisoning
(Saxitoxin analogues): Biosynthesis, Analytical methods, Dynamics
4. Amnesic shellfish poisoning (Domoic
acids): Biosynthesis, Natural analogues
5. Electrophysiological study of mode
of action of bioactive Natural Products.
6. Seaweed toxins (polycavernoside A)
: Isolation and the structures of congeners, Origin, Analytical methods,
Pharmacology
7. Bioactive compounds from marine
animals : isolation, structure elucidation and biological activities of the
novel compounds.
8. Toxins from amphibians :
tetrodotoxin and its congeners, and zetekitoxin (an analogue of saxitoxin)
Members
Professor : Mari Yotsu-Yamashita, Ph.D.
{publication list: see bellow}
TEL/FAX 81-22-757-4425 Email:
mari.yamashita.c1@[please add tohoku.ac.jp after @]
Associate professor: Keiichi Konoki,
Ph.D. {publication list: see bellow} [publication
list in previous laboratory]
Email:keiichi.konoki.b2@ [please add
tohoku.ac.jp after @]
Assistant
professor: Yuko Cho, Ph.D. {publication list: see bellow} [publication list in previous laboratory]
Email: yuko.cho.a4@ [please
add tohoku.ac.jp after @]
Associate professor (Frontier Research
Institute for Interdisciplinary Science (FRIS), Creative Interdisciplinary
Research Division)):
Yuta Kudo, Ph.D. {publication list: see bellow} [publication list in previous laboratory]
TEL 81-22-757-4427 FAX 81-22-757-4425
Email: yuta.kudo.d5@ [please add tohoku.ac.jp after @]
Graduate students:
Ryosuke Hirozumi (D3) JST spring fellow
Ranmaru Matsui (M2)
Masaki Mochizuki (M2)
Takumi Hasegawa (M2)
Masashi Gamo (M1)
Ayami Sato (M1)
Yuto Yoshida (M1)
Under graduate students:
Natsumi Takahara (B4)
Shugo Horie (B4)
Yuya Yoshida (B4)
Runa Yonekura (B4)
Publication list (1999-) *corresponding author
Tadachika Miyasaka, Yuta Kudo, Mari Yostu-Yamashita, Masaatsu Adachi, Toshio Nishikawa*, Synthetic Studies Toward 4,9-Anhydro-10-hemiketal-5-deoxy-tetrodotoxin, A Proposed Biosynthetic Precursor of Tetrodotoxin, SYNLETT, accepted, 2025, 4th April.
Y. Kudo*, K. Konoki and M.
Yotsu-Yamashita, Identification of γ-Butyrolactone Signalling Molecules in Diverse Actinomycetes Using
Resin-Assisted Isolation and Chemoenzymatic Synthesis, RSC Chem. Biol., 2025, 6,
630–641. https://doi.org/10.1039/D5CB00007F
(open access).
Ryosuke Hirozumi, Yuta Kudo, Yuko
Cho, Keiichi Konoki, Mari Yotsu-Yamashita*, Total Synthesis and Structural
Revision of (±)-Mauritamide B, J. Nat.
Prod., 2025, 88, 3,
806–814. https://doi.org/10.1021/acs.jnatprod.5c00019
(open access).
Mari Yotsu-Yamashita*, Kairi
Umeda, Arihiro Iwasaki, Kiyotake Suenaga, Toxicity of the marine glycosidic
macrolides, polycavernoside E and akunolides, from Okinawan cyanobacterium
Okeania sp. in mice, Toxicon 2024,
108122-108122, (open access), https://doi.org/10.1016/j.toxicon.2024.108122.
Yuko Cho*, Shizu Hidema, Takuo
Omura, Shigeki Tsuchiya, Keiichi Konoki, Yasukatsu Oshima, Mari
Yotsu-Yamashita, Intracellular abundance, localization, and enzymatic activity
of a saxitoxin biosynthesis enzyme, SxtG, in two sister subclones of the
dinoflagellate Alexandrium catenella with extremely different levels of
paralytic shellfish toxins, Harmful Algae
2024, 139, 102723-102723 (open access), https://doi.org/10.1016/j.hal.2024.102723.
Ryosuke Hirozumi, Mayu Hakamada,
Takashi Minowa, Yuko Cho, Yuta Kudo, Keiichi Konoki, Yasukatsu Oshima, Kazuo
Nagasawa, Mari Yotsu-Yamashita*, Synthesis of Saxitoxin Biosynthetic
Intermediates: Reveal the Mechanism for Formation of its Tricyclic Skeleton in
Biosynthesis, Chemistry - An Asian
Journal, 2024, e202400834 (open access),
https://doi.org/10.1002/asia.202400834.
Masato Terauchi,†, Yuki
Komazaki,†, Atsushi Yoshino, Yuko Cho, Yuta Kudo, Mari Yotsu-Yamashita,
Keiichi Konoki∗,†These authors contributed equally to this work. Assay for okadaic acid O-acyl transferase using HPLC-FLD, Bioscience, Biotechnology, and Biochemistry,
2024, 88, 99-1006. https://doi.org/10.1093/bbb/zbae085
Tatsunari Shingai*, Yoshiko Chiba,
Mitsue Kondo, Mari Yotsu-Yamashita, Temporal variation in the
concentrations and profiles of paralytic shellfish toxins and tetrodotoxin in
scallop (Mizuhopecten yessoensis) and
bloody clam (Anadara broughtonii)
collected from the coast of Miyagi Prefecture, Japan, Toxicon, 2024,107710. https://doi.org/10.1016/j.toxicon.2024.107710.
M. Hakamada‡, C.
Tokairin‡, H. Ishizuka‡, K. Adachi, T. Osawa, S. Aonuma,
R. Hirozumi, S. Tsuchiya, Y. Cho, Y. Kudo, K. Konoki, Y. Oshima, K. Nagasawa*,
M. Yotsu-Yamashita*, Synthesis and Identification of decarbamoylosySaxitoxins
in Toxic Microalgae and their Reaction with the Oxygenase, SxtT, Reveal
Saxitoxin Biosynthesis, Chem. Eur. J.
e202304238 (2024). http://dx.doi.org/10.1002/chem.202304238
‡These authors contributed equally
to this work. (Open access).
Hayate Ishizuka, Ayato Nureki,
Kanna Adachi, Yuka Takayanagi, Minami Odagi, Mari Yotsu-Yamashita, and
Kazuo Nagasawa*, Approaches to Construction of the Medium-Sized Ring Structure
in Zetekitoxin AB by Ring-Closing Metathesis, J. Org. Chem. 2023, 88,
14, 10223–10231. https://pubs.acs.org/doi/10.1021/acs.joc.3c00580
Shota Itoda, Ryuichi Watanabe,
Mayu Ozawa, Hajime Uchida, Ryoji Matsushima, Hiroshi Oikawa, Hideto Fukushima,
Masahiro Matsumiya, Mari Yotsu-Yamashita and Toshiyuki Suzuki*, Chemical analysis of
tetrodotoxins in foods made using pufferfish ovaries pickled in salt and
rice-bran, Nippon Suisan Gakkaishi, 2023,
89 (3), 244-249. DOI: 10.2331/suisan.22-00051
Ayato Nureki,
Kanna Adachi, Hayate Ishizuka, Kako Maeno, Yayoi Orihara, Minami Odagi, Mari
Yotsu-Yamashita, Kazuo Nagasawa*, Stereoselective Construction of Tertiary
Alcohol at C11 of Zetekitoxin AB, Asian
J. Org. Chem., 2023, e202300205, https://doi.org/10.1002/ajoc.202300205
Keita Yamaki,
Kyoka Sato, Yuta Kudo, Yuko Cho, Keiichi Konoki, Tomohiro Takatani, Osamu Arakawa,
Kentaro Kawatsu*, and Mari Yotsu-Yamashita*, The quite low
cross-reactivity of Kawatsu’s anti-tetrodotoxin monoclonal antibody to
5,6,11-trideoxytetrodotoxin, 11-nortetrodotoxin-6(S)-ol, and 11-oxotetrodotoxin, the major tetrodotoxin analogues in
pufferfish, Toxicon, 2023,
226, 107081. https://doi.org/10.1016/j.toxicon.2023.107081
Yuko Cho*,
Shigeki Tsuchiya, Takuo Omura, Kazuhiko Koike, Keiichi Konoki,
Yasukatsu Oshima, Mari Yotsu-Yamashita. Metabolic inhibitor induces
dynamic changes in saxitoxin biosynthesis and metabolism in the dinoflagellate Alexandrium
pacificum (Group IV) under in vivo labeling condition, Harmful
Algae, 2023, 122, 102372. https://doi.org/10.1016/j.hal.2022.102372
Osamu Chiba1, Noriko Shimada1, Shutaro Yoshio, Yuta
Kudo, Yuko Cho, Mari Yotsu-Yamashita, Keiichi Konoki*, State-Dependent
Inhibition of Voltage-Gated Sodium Channels in Neuroblastoma Neuro-2A Cells by
Arachidonic Acid from Halichondria okadai, Chemical Research in
Toxicology, 2022, 35, 11, 1950-1961. 1These
authors contributed equally to this work. https://pubs.acs.org/doi/10.1021/acs.chemrestox.2c00062
Ryoma Minami, Kaori Taniguchi, Hironobu Matsuo, Kazunari Tsujimura, Ryoma
Miura, Yusuke Shibahara, Kentaroi Kawatsu, Mari Yotsu-Yamashita,
Tomohiro Takatani, Osamu Arakawa*, Screening method for tetrodotoxin using
immunochromatographic test kit, Nippon Suisan Gakkaishi, 2022, 88(6)
515-522. DOI: 10.2331/suisan.22-00031
Yuji Yaegashi, Yuta Kudo, Nozomi
Ueyama, Ken-ichi Onodera, Yuko Cho, Keiichi
Konoki, Mari Yotsu-Yamashita*, Isolation and biological activity of 9-epiTetrodotoxin, and isolation of
Tb-242B, possible biosynthetic shunt products of tetrodotoxin from pufferfish, J. Nat. Prod. 2022, 85, 2199–2206. https://pubs.acs.org/doi/10.1021/acs.jnatprod.2c00588.
Gorapava, C.; Nih, S.; Maeno, Y.;
Pin, C.; Teen, P.; Yotsu-Yamashita, M.; Terada, R.; Kotaki, Y.* First
record of the diatom Nitzschia
Navis-varingica ( Bacillariophyceae ) producing Amnesic Shellfish
Poisoning-Toxins from Papua New Guinea. Toxicon
2022, 216, 65–72. https://doi.org/10.1016/j.toxicon.2022.06.016
Atsuo Nakazaki,* Shunsuke Mouri, Yoshiki
Nakane, Yuki Ishikawa, Mari Yotsu-Yamashita, and Toshio Nishikawa, The
synthesis of simplified analogues of crambescin b carboxylic acid and their
inhibitory activity of voltage-gated sodium channels: new aspects of
structure–activity relationships, Heterocycles
2022, 105, 343–351. DOI: 10.3987/COM-21-S(R)7
Yuta Kudo*, Keiichi Konoki, Mari Yotsu-Yamashita*, Mass spectrometry–guided discovery of new
analogues of bicyclic phosphotriester salinipostin and evaluation of their
monoacylglycerol lipase inhibitory activity, Bioscience, Biotechnology, and Biochemistry, 2022, 86,
1333–1342. https://doi.org/10.1093/bbb/zbac131
Watanabe, R.; Takayanagi, Y.; Chiba, O.;
Itoda, S.; Ishizuka, H.; Odagi, M.; Ozawa, M.; Uchida, H.; Matsushima, R.; Konoki,
K.; Yotsu-Yamashita*, M.; Nagasawa*, K.; Suzuki*, T. Nontoxic
Enantiomeric Reference Materials for Saxitoxins. 2022. Analytical Chemistry, 94, 32,
11144–11150. https://doi.org/10.1021/acs.analchem.2c00836
Michiru Akamatsu1, Ryosuke Hirozumi1, Yuko Cho,
Yuta Kudo, Keiichi Konoki, Yasukatsu Oshima, Mari Yotsu-Yamashita*, First
Identification of 12β-Deoxygonyautoxin 5 (12α-Gonyautoxinol 5) in the
Cyanobacterium Dolichospermum circinale
(TA04) and 12β-Deoxysaxitoxin (12α-Saxitoxinol) in D. circinale (TA04) and the
Dinoflagellate Alexandrium pacificum
(Group IV) (120518KureAC), Marine Drugs,
2022, 20(3) 166. https://doi.org/10.3390/md20030166.
1These authors contributed equally to this work.
Taylor S. Steele1,
John K. Brunson1, Yukari Maeno, Ryuta Terada, Andrew E.
Allen, Mari Yotsu-Yamashita, Jonathan, R. Chekan*, Bradley S. Moore*,
Domoic acid biosynthesis in the red alga Chondria
armata suggests a complex evolutionary history for toxin production, Proc. Natl. Acad. Sci. U.S.A., 2022,
119(6) e2117407119.
https://doi.org/10.1073/pnas.2117407119.
1These authors contributed equally to this work.
Dietrich Mebs,*, Julio V. Schneider, Oskar Schr¨oder, Mari
Yotsu-Yamashita, John R. Harley, Linda Mogk, Gunther K¨ohler, A study on
the genetic population structure and the tetrodotoxin content of rough-skinned
newts, Taricha granulosa
(Salamandridae), from their northern range of distribution, Toxicon 206 (2022) 38–41. https://doi.org/10.1016/j.toxicon.2021.12.010
Yukari Maeno, Yuichi Kotaki, Ryuta Terada, Masafumi
Hidaka, Yuko Cho, Keiichi Konoki, and Mari Yotsu-Yamashita*, Preparation of domoic acid analogues using a
bioconversion system, and their toxicity in mice, online published, Organic & Biomolecular Chemistry,
2021, 19, 7894-7902.
https://doi.org/10.1039/D1OB01378E
Dietrich Mebs* and Mari
Yotsu-Yamashita, Acquiring toxicity of a newt, Cynops orientalis, Toxicon,
2021, 198, 32–35.
https://doi.org/10.1016/j.toxicon.2021.04.025
Yuta Kudo,
Charles T. Hanifin, and Mari Yotsu-Yamashita*, Identification of
Tricyclic Guanidino Compounds from the Tetrodotoxin-Bearing Newt Taricha granulosa, Org. Lett., 2021,
23, 3513-3517.
https://doi.org/10.1021/acs.orglett.1c00916
Two new skeletal analogues of saxitoxin found in the
scallop, Patinopecten yessoensis, as
possible metabolites of paralytic shellfish toxins, Satoshi Numano, Yuta
Kudo, Yuko Cho, Keiichi Konoki, Yoshimasa Kaga, Kazuo Nagasawa, and Mari
Yotsu-Yamashita*, Chemosphere,
2021, 278, 130224.
https://doi.org/10.1016/j.chemosphere.2021.130224
Keisuke Nishikawa,* Takayuki Noguchi, Seiho Kikuchi,
Takahiro Maruyama, Yusuke Araki, Mari Yotsu-Yamashita, and Yoshiki
Morimoto*, Tetrodotoxin Framework Construction from Linear Substrates Utilizing
a Hg(OTf)2‐Catalyzed
Cycloisomerization Reaction: Synthesis of the Unnatural Analogue
11-nor-6,7,8-Trideoxytetrodotoxin, Organic
Letters, 2021, 23, 1703-1708.
https://dx.doi.org/10.1021/acs.orglett.1c00125
Mari Yotsu-Yamashita, Gunther Köhler, Dietrich Mebs,* Polypedates Leucomystax (White-lipped
Tree Frog) Toxicity. Herpetological
Review, 51(4), 2020, 822-823.
Yuko Cho*, Shizu Hidema, Takuo Omura, Kazuhiko
Koike, Kanae Koike, Hiroshi Oikawa, Keiichi Konoki, Yasukatsu Oshima,
and Mari Yotsu-Yamashita, SxtA localizes to chloroplasts and changes to
its 3’UTR may reduce toxin biosynthesis in non-toxic Alexandrium catenella (Group
I), Harmful Algae, 101, 101972, 2021.
Invited Feature Article.
http://doi.org/10.1016/j.hal.2020.101972
Yuta Kudo, Charles Hanifin, Yuichi Kotaki, Mari
Yotsu-Yamashita*, Structures of N-hydroxy-type
tetrodotoxin analogues and bicyclic guanidinium compounds found in toxic newts, Journal of Natural Products, 2020, 83,
9, 2706-2717. ACS Editor’s Choice. (Open
access)
https://pubs.acs.org/doi/abs/10.1021/acs.jnatprod.0c00623
Dietrich Mebs*, Mari Yotsu-Yamashita, Katharina
Hartmann, Christine Elbert, Richard Zehner, Stefan W. Toennes
Revisited - Failure of tetrodotoxin to protect
red-spotted newts, Notophthalmus
viridescens, from endoparasites, Toxicon,
2020, 178, 77–81.
https://doi.org/10.1016/j.toxicon.2020.02.026
Kanna Adachi,‡ Tomoshi Yamada,‡ Hayate
Ishizuka, Mana Oki, Shunsuke Tsunogae, Noriko Shimada, Osamu Chiba,
Tatsuya Orihara, Masafumi Hidaka, Takatsugu Hirokawa, Minami Odagi, Keiichi
Konoki,* Mari Yotsu-Yamashita,* Kazuo Nagasawa* (‡ contributed equally to
this work), Synthesis of C12‐keto saxitoxin derivatives with unusual inhibitory
activity against voltage‐gated sodium channels, Chem. Eur. J., 2020, 26, 2025-2033.
https://doi.org/10.1002/chem.201904184
Ryuichi Watanabe, Masato Tanioka, Hajime Uchida, Ryoji
Matsushima, Hiroshi Oikawa,Masahiro Matsumiya, Mari Yotsu-Yamashita, and
Toshiyuki Suzuki*, Quantitation of Tetrodotoxin and Its Analogues with a
Combination of Liquid Chromatography−Tandem Mass Spectrometry and Quantitative 1H
NMR Spectroscopy, J. Agric. Food Chem.
2019, 67, 46, 12911-12917.
http://pubs.acs.org/doi/abs/10.1021/acs.jafc.9b06380
Satoshi Numano, Yuta Kudo, Yuko Cho, Keiichi Konoki
and Mari Yotsu-Yamashita,* Temporal Variation of the Profile and Concentrations
of Paralytic Shellfish Toxins and Tetrodotoxin in the Scallop, Patinopecten yessoensis, Cultured in a
Bay of East Japan, Mar. Drugs 2019,17(12), 653; https://doi.org/10.3390/md17120653
(open access)
Keiichi Konoki*, Daniel G. Baden, Todd Scheuer and W liam
A. Catterall, Molecular Determinants of Brevetoxin Binding to Voltage-Gated
Sodium Channels, Toxins 2019, 11(9),
513. http://doi.org/10.3390/toxins11090513
(open access)
Takashi Minowa, Yuko Cho, Yasukatsu Oshima, Keiichi
Konoki and Mari Yotsu-Yamashita*, Identification of a Novel Saxitoxin
Analogue, 12β-Deoxygonyautoxin 3, in the
Cyanobacterium, Anabaena circinalis
(TA04), Toxins 2019, 11(9), 539. http://doi.org/10.3390/toxins11090539
(open access)
Yukari Maeno, Ryuta Terada, Yuichi Kotaki, Yuko Cho,
Keiichi Konoki, and Mari Yotsu-Yamashita*, Possible biosynthetic products
and metabolites of kainic acid from the red alga, Digenea simplex, and their
biological activity, Journal of Natural
Products, 2019, 82, 1627-1633. https://pubs.acs.org/doi/10.1021/acs.jnatprod.9b00128
Yuta Kudo and Mari Yotsu-Yamashita*,
Isolation and biological activity of 8-epitetrodotoxin
and the structure of a possible biosynthetic shunt product of tetrodotoxin,
Cep-226A, from the newt, Cynops ensicauda
popei, Journal of Natural Products,
2019, 82, 1656-1663. https://pubs.acs.org/doi/10.1021/acs.jnatprod.9b00178
Yuko Cho*, Shigeki
Tsuchiya, Takuo Omura, Kazuhiko Koike, Hiroshi Oikawa, Keiichi Konoki,
Yasukatsu Oshima, Mari Yotsu-Yamashita, Metabolomic study of saxitoxin
analogues and biosynthetic intermediates in dinoflagellates using 15N-labelled
sodium nitrate as a nitrogen source, Scientific
Reports, 2019, 9: 3460. https://www.nature.com/articles/s41598-019-39708-y
Masaatsu Adachi*, Tadachika Miyasaka, Yuta Kudo, Keita Sugimoto, Mari
Yotsu-Yamashita, and Toshio Nishikawa*, Total Syntheses and Determination
of Absolute Configurations of Cep-212 and Cep-210, Predicted Biosynthetic
Intermediates of Tetrodotoxin Isolated from Toxic Newt, Organic Letters, 2019, 21, 780-784. https://pubs.acs.org/doi/full/10.1021/acs.orglett.8b04043
Dietrich Mebs*, Mari Yotsu-Yamashita, Stefan
W. Toennes, Tetrodotoxin content of Rough-skinned Newts, Taricha granulosa
(Salamandridae), from their northern distribution range, British Columbia,
Canada, and Southeast-Alaska, USA, Salamandra 55(2), 82-88, 2019. ISSN 0036-3375 http://www.salamandra-journal.com/index.php/home/contents/2019-vol-55/
- Volume55-2
Andrew J. Jang*, Ji-Hyeok Lee, Mari
Yotsu-Yamashita, Joodong Park, Steve Kye, Raymond L. Benza, Michael J.
Passineau, You-Jin Jeon & Toru Nyunoya A Novel Compound, “FA-1” Isolated from
Prunus mume, Protects Human Bronchial Epithelial Cells and Keratinocytes from
Cigarette Smoke Extract-Induced Damage, Scientific
Reports, 8, 11504, 2018.
https://doi.org/10.1038/s41598-018-29701-2
Dietrich Mebs*, Mari
Yotsu-Yamashita, Werner Pogoda, Joseph Vargas Alvarez, Raffael Ernst,
Gunther Köhler, Stefan W. Toennes, Lack of alkaloids and tetrodotoxin in the
neotropical frogs Allobates spp.
(Aromobatidae) and Silverstoneia flotator (Dendrobatidae)
Toxicon
152, 103–105, 2018.
https://doi.org/10.1016/j.toxicon.2018.07.027
Mari Yotsu-Yamashita*, Yuuma Nagaoka,
Koji Muramoto, Yuko Cho and Keiichi Konoki, Pufferfish Saxitoxin and Tetrodotoxin
Binding Protein (PSTBP) Analogues in the Blood Plasma of the Pufferfish Arothron nigropunctatus, A. hispidus, A.
manilensis, and Chelonodon patoca,
Mar. Drugs 2018, 16(7), 224.
https://doi.org/10.3390/md16070224
Sou Kikuchi1, Kayo Okada1, Yuko Cho, Shinichiro Yoshida, Eunsang Kwon, Mari Yotsu-Yamashita, Keiichi Konoki*, Isolation and structure determination of lysiformine from bacteria associated with marine sponge Halichondria okadai, Tetrahedron 2018, 74, 3742-3747. (1 These authors contributed equally to this work.)
https://dx.doi.org/10.1016/j.tet.2018.05.049
Dietrich Mebs∗, Max Lorentz, Mari Yotsu-Yamashita, Daniela C. Rößler, Raffael Ernst, Stefan Lötters, Geographic range expansion of tetrodotoxin in amphibians – First record
in Atelopus hoogmoedi from the Guiana Shield, Toxicon, 2018, 150, 175-179.
https://dx.doi.org/10.1016/j.toxicon.2018.05011
Nozomi Ueyama, Keita Sugimoto, Yuta Kudo, Ken-ichi Onodera, Yuko Cho, Keiichi Konoki, Toshio Nishikawa, Mari Yotsu-Yamashita*, Spiro Bicyclic Guanidino Compounds from Pufferfish, Possible Biosynthetic Intermediates of Tetrodotoxin in Marine Environments, Chemistry, a European Journal, 2018, 24, 7250-7258.
http://dx.doi.org/10.1002/chem.201801006.
Yukari
Maeno, Yuichi Kotaki, Ryuta Terada, Yuko
Cho, Keiichi Konoki, Mari Yotsu-Yamashita*,
Six domoic acid related compounds from the red alga, Chondria armata,
and domoic acid biosynthesis by the diatom, Pseudo-nitzschia multiseries,
Scientific Reports | (2018) 8:356 DOI:10.1038/s41598-017-18651-w
Open
access: www.nature.com/articles/s41598-017-18651-w
Dietrich Mebs* and Mari
Yotsu-Yamashita, Taricha granulosa
(Rough-Skinned Newt) Predation, Herpetological Review, 2017, 48(4), 828-829.
Kotaro Iwasaki, Satori Sasaki, Yusuke
Kasai, Yuki Kawashima, Shohei Sasaki, Takanori Ito, Mari
Yotsu-Yamashita, and Makoto Sasaki,* Total
Synthesis of Polycavernosides A and B, Two Lethal Toxins from Red Alga. The
Journal of Organic Chemistry, 82, 13204-13219 (2017). DOI:10.1021/acs.joc.7b02293
Tadaaki
Tsukamoto, Yukie Chiba,
Minoru Wakamori, Tomoshi Yamada,
Shunsuke Tsunogae, Yuko
Cho, Ryo Sakakibara, Takuya Imazu, Shouta
Tokoro, Yoshiki Satake, Masaatsu Adachi, Toshio Nishikawa, Mari
Yotsu-Yamashita, and Keiichi
Konoki,* Differential binding of tetrodotoxin
and its derivatives to voltage-sensitive sodium channel subtypes (Nav1.1 to
Nav1.7), British Journal of Pharmacology, 2017, 174, 3811-3892. http://onlinelibrary.wiley.com/doi/10.1111/bph.13985/full
Yuta
Kudo, Chikafumi Chiba, Keiichi
Konoki, Yuko Cho and Mari
Yotsu-Yamashita*, Dietary administration of
tetrodotoxin and its putative biosynthetic intermediates to the captive-reared
non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster. Toxicon,
2017, 134, 78-82. https://doi.org/10.1016/j.toxicon.2017.07.016
Yotsu-Yamashita,
M., Toennes, S.W., Mebs, D.*,
Tetrodotoxin in Asian newts (Salamandridae), Toxicon, 2017, 134, 14-17.
doi:
10.1016/j.toxicon.2017.05.014.
Shigeki
Tsuchiya, Yuko Cho, Renpei Yoshioka, Keiichi Konoki, Kazuo
Nagasawa, Yasukatsu Oshima, and Mari Yotsu-Yamashita*,
Synthesis and identification of key biosynthetic intermediates for the
formation of the tricyclic skeleton of saxitoxin, Angew. Chem. Int. Ed.,
2017, 56, 5327-5331.
http://onlinelibrary.wiley.com/doi/10.1002/anie.201612461/full
See
also: http://www.chemistryviews.org/details/ezine/10513592/Biosynthesis_of_Saxitoxin.html
Tadaaki
Tsukamoto, Yukie Chiba, Atsuo
Nakazaki, Yuki Ishikawa, Yoshiki Nakane, Yuko Cho, Mari
Yotsu-Yamashita, Toshio
Nishikawa, Minoru Wakamori, and Keiichi Konoki,*
Inhibition of veratridine-induced delayed inactivation of the voltage-sensitive
sodium channel by synthetic analogs of crambescin B, Bioorganic &
Medicinal Chemistry Letters, 2017, 27, 1247-1251. http://dx.doi.org/10.1016/j.bmcl.2017.01.054
Clyde
Gorapava Puilingi, Yuta Kudo, Yuko Cho, Keiichi Konoki, and Mari
Yotsu-Yamashita*, A new sarasinoside congener,
sarasinoside M2, from a
marine sponge collected in the Solomon Islands, Bioscience, Biotechnology,
and Biochemistry, 2017, 81, 222-225. http://dx.doi.org/10.1080/09168451.2016.1246172
Mebs, D.,* M.
Yotsu-Yamashita, J. Ream, B. K. Zajac & R.
Zehner: Tetrodotoxin concentrations in rough-skinned newts, Taricha
granulosa, from populations of their northern distribution range. Sarlamandra,
52(3), 255-206 (2016).
http://www.salamandra-journal.com/index.php?option=com_docman&Itemid=80
Yuko
Cho*, Shigeki Tsuchiya, Renpei Yoshioka,
Takuo Omura, Keiichi Konoki, Yasukatsu
Oshima, Mari Yotsu-Yamashita,
Column switching combined with hydrophilic interaction chromatography-tandem
mass spectrometry for the analysis of saxitoxin analogues, and their
biosynthetic intermediates in dinoflagellates. Journal of Chromatography A, 2016,
1474, 109-120. http://dx.doi.org/10.1016/j.chroma.2016.10.065
Yu Onodera, Kazuaki Hirota, Yuto Suga, Keiichi
Konoki, Mari Yotsu-Yamashita,
Makoto Sasaki, and Haruhiko Fuwa*, Diastereoselective Ring-Closing Metathesis
as a Means to Construct Medium-Sized Cyclic Ethers: Application to the
Synthesis of a Photoactivatable Gambierol Derivative, J. Org. Chem.,
2016, 81 (18), 8234_8252, 2016. DOI:
10.1021/acs.joc.6b01302
Chao Wang, Mana
Oki,
Toru Nishikawa, Daisuke
Harada, Mari Yotsu-Yamashita
and Kazuo Nagasawa*, Total Synthesis of 11-Saxitoxinethanoic Acid and
Evaluation of its Inhibitory Activity on Voltage-gated Sodium Channels, Angew.
Chem. Int. Ed., 55 (38), 11600_11603, 2016. DOI: 10.1002/anie.201604155
Naoyuki Umemoto*, Masaru Nakayasu,
Kiyoshi Ohyama, Mari Yotsu-Yamashita,
Masaharu Mizutani, Hikaru Seki, Kazuki Saito, and Toshiya Muranaka, Two
Cytochrome P450 Monooxygenases Catalyze Early Hydroxylation Steps in the Potato
Steroid Glycoalkaloid Biosynthetic Pathway. Plant Physiol. 171,
2458-2467, 2016.; doi:10.1104/pp.16.00137
Atsuo Nakazaki, Yoshiki Nakane, Yuki
Ishikawa, Mari Yotsu-Yamashita
and Toshio Nishikawa*, Asymmetric synthesis of crambescin A_C carboxylic acids
and their inhibitory activity on voltage-gated sodium channels, Org. Biomol.
Chem., 14, 5304-5309, 2016, DOI: 10.1039/c6ob00914j
Shogo
Saruhashi, Keiichi Konoki, and Mari Yotsu-Yamashita*,
The voltage-gated sodium ion channel inhibitory activities of a new
tetrodotoxin analogue, 4,4a-anhydrotetrodotoxin, and three other analogues
evaluated by colorimetric cell-based assay, Toxicon, 119, 72-76, 2016. doi:10.1016/j.toxicon.2016.05.012
Sachie
Furumochi, Tatsuya Onoda, Yuko Cho, Haruhiko
Fuwa, Makoto Sasaki, Mari Yotsu-Yamashita, and
Keiichi Konoki*, Effect of carbon chain length
in acyl coenzyme A on the efficiency of enzymatic transformation of okadaic
acid to 7-O-acyl okadaic acid, Bioorg. Med. Chem. Lett., 26,
2992-2996, 2116. doi:10.1016/j.bmcl.2016.05.027
Yuta
Kudo, Takeshi Yasumoto, Dietrich Mebs, Yuko
Cho, Keiichi Konoki,
and Mari Yotsu-Yamashita*,
Cyclic Guanidine Compounds From Newts Support the Hypothesis that Tetrodotoxin
is Derived From Monoterpene. Angew. Chem. Int. Ed., 55, 8728-8731, 2016.
DOI:10.1002/anie.201602971, Angew. Chem. 128 (30),
8870_8873. DOI: 10.1002/ange.201602971.
Davide Romani, Shigeki
Tsuchiya, Mari Yotsu-Yamashita, Silvia A.
Brand_n*, Spectroscopic and structural investigation on intermediates species
structurally associated to the tricyclic bisguanidine compound and to the toxic
agent, saxitoxin, Journal of Molecular Structure, 2016, 1119, 25-38.
doi:10.1016/j.molstruc.2016.04.039
http://www.sciencedirect.com/science/article/pii/S0022286016303581
.
Dietrich Mebs*, Mari
Yotsu-Yamashita and Osamu Arakawa, The praying
mantis (Mantodea) as predator of the poisonous red-spotted newt
Notophthalmus viridescens
(Amphibia: Urodela: Salamandridae), Chemoecology, 2016, http://link.springer.com/article/10.1007/s00049-016-0211-3
Shigeki
Tsuchiya, Yuko Cho, Keiichi Konoki, Kazuo
Nagasawa, Yasukatsu Oshima and Mari Yotsu-Yamashita,* Biosynthetic
route towards saxitoxin and shunt pathway. Scientific Reports, 2016,
6:20340 | DOI: 10.1038/srep20340. http://www.nature.com/articles/srep20340
(Open access)
Keiichi
Konoki*, Kayo Okada, Mami
Kohama, Hiroki Matsuura,
Kaori Saito, Yuko Cho, Goh
Nishitani, Tomofumi Miyamoto, Seketsu Fukuzawa, Kazuo Tachibana, Mari
Yotsu-Yamashita, Identification of okadaic acid
binding protein 2 in reconstituted sponge cell clusters from Halichondria
okadai and its contribution to the detoxification of okadaic acid. Toxicon,
2015, 108, 38-45. doi:10.1016/j.toxicon.2015.09.026
Yuko
Cho*, Shigeki Tsuchiya, Renpei Yoshioka, Takuo
Omura, Keiichi Konoki,
Yasukatsu Oshima and Mari Yotsu-Yamashita,
The presence of 12-deoxydecarbamoylsaxitoxin in the Japanese toxic dinoflagellate
Alexandrium determined by simultaneous analysis for paralytic shellfish toxins
using HILIC-LC-MS/MS, Harmful Algae, Harmful Algae, 2015, 49,
58-67. doi:10.1016/j.hal.2015.09.003
Clyde
Gorapava Puilingi, Yuta Kudo, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita*,
Tetrodotoxin and its analogues in the pufferfish Arothron hispidus and A.
nigropunctatus from the Solomon Islands: A comparison of their toxin
profiles with the same species from Okinawa, Japan, Toxins, 2015, 7,
3436-3454.
http://www.mdpi.com/2072-6651/7/9/3436/pdf (open
access)
Ehara H, Makino M, Kodama K, Konoki
K, Ito T, Sekine S, Fukuzawa S*,
Yokoyama S*, Tachibana K*, Crystal Structure of Okadaic Acid Binding Protein
2.1: A Sponge Protein Implicated in Cytotoxin Accumulation. ChemBioChem.
2015, 16(10):1435-9.
Yuta
Kudo, Chikafumi Chiba, Yuko
Cho, Keiichi Konoki, Mari Yotsu-Yamashita*,
Confirmation of the absence of tetrodotoxin and its analogues in the juveniles
of the Japanese fire-bellied newt, Cynops pyrrhogaster, captive-reared
from eggs in the laboratory using HILIC-LC-MS, Toxicon, 2015, 101,
101-105.
doi:10.1016/j.toxicon.2015.05.008
Shigeki
Tsuchiya, Yuko Cho,
Keiichi Konoki, Kazuo
Nagasawa, Yasukatsu Oshima, and Mari Yotsu-Yamashita*,
Synthesis of a tricyclic bisguanidine compound structurally related to
saxitoxin and its identification in paralytic shellfish toxins-producing
microorganisms, Chem. Eur. J. 2015, 21, 7835-7840.
see
also: http://www.chemistryviews.org/details/ezine/7892661/A_Pathway_to_Saxitoxin.html.
Noriyoshi Teramoto* and Mari
Yotsu-Yamashita, Selective Blocking Effects of
4,9-Anhydrotetrodotoxin, Purified from a Crude Mixture of Tetrodotoxin
Analogues, on NaV1.6, Channels
and Its Chemical Aspects, Marine Drugs 13, 984-995, 2015 (Review)
(open access: http://www.mdpi.com/1660-3397/13/2/984.)
(Review).
Keiichi
Konoki,*, Yuto Suga, Haruhiko Fuwa, Mari
Yotsu-Yamashita, and Makoto Sasaki,* Evaluation
of gambierol and its analogs for their inhibition of human Kv1.2 and
cytotoxicity, Bioorg. Med. Chem. Lett., 2014, 25, 514-518.
DOI:10.1016/j.bmcl.2014.12.022
Nicolaou, K. C.*, Heretsch, P.,
Nakamura, T., Rudo A., Murata, M, and Konoki, K.
Synthesis and Biological Evaluation of QRSTUVWXYZA' Domains of Maitotoxin. J
Am Chem Soc., 2014, 136, 16444_16451.
Kunitake, M., Oshima, T., Konoki,
K., Ebine, M., Torikai, K., Murata, M.,
and Oishi, T.* Synthesis and biological activity of the C'D'E'F' ring system of
maitotoxin. J Org Chem. 2014, 79, 4948-4962. doi: 10.1021/jo5005235.
Onoue, H., Baba, T., Konoki,
K., Torikai, K., Ebine, M., and Oishi,
T.* Synthesis and Biological Activity of the QRS Ring System of Maitotoxin. Chem.
Lett. 2014, 43, 1904-1906.
Yuta
Kudo, Yoko Yamashita,
Dietrich Mebs, Yuko Cho, Keiichi
Konoki, Takeshi Yasumoto, and Mari
Yotsu-Yamashita*, C5_C10 Directly bonded
tetrodotoxin analogues: Possible biosynthetic precursors of tetrodotoxin from
newts. Angew. Chem. Int. Ed. 2014, 53, 14546-14549.
.
Yoshiki Satake, Masaatsu Adachi, Shouta
Tokoro, Mari Yotsu-Yamashita,
Minoru Isobe, and Toshio Nishikawa*, Synthesis of 5- and 8-Deoxytetrodotoxin, Chem.
Asian J. 2014, 9, 1922 _ 1932.
Yuma
Kudo, Akihiro Abe,
Kumiko Ito, Yuko Cho, Mari
Yotsu-Yamashita, and Keiichi
Konoki*, Expression of recombinant alpha and
beta tubulins from the yew Taxus cuspidata and analysis of the
microtubule assembly in the presence of taxol, Bioscience, Biotechnology,
and Biochemistry, accepted (9th June, 2014).
Shigeki
Tsuchiya, Yuko Cho,
Keiichi Konoki, Kazuo
Nagasawa, Yasukatsu Oshima and Mari Yotsu-Yamashita*,
Synthesis and identification of proposed biosynthetic intermediates of
saxitoxin in the cyanobacterium Anabaena circinalis (TA04) and the
dinoflagellate Alexandrium tamarense (Axat-2), Org. Biomol. Chem.,
12, 3016-3020, 2014.
Yuta
Kudo, Julian Finn, Kohei Fukushima, Satsuki
Sakugawa, Yuko Cho, Keiichi
Konoki, and Mari Yotsu-Yamashita*,
Isolation of 6-deoxytetrodotoxin from the pufferfish, takifugu pardalis, and a
comparison of the effects of the C-6 and C-11 hydroxy groups of tetrodotoxin on
its activity, J. Nat. Prod., 77, 1000-1004, 2014.
Mori, Tadashi; HIDAKA, Masafumi; Ikuji,
Hiroko; Yoshizawa, Ibuki; TOYOHARA, Haruhiko; Okuda, Toru; Uchida, Chiyoko;
Asano, Tomoichiro; Yotsu-YAMASHITA, Mari;
UCHIDA, Takafumi*, A High-Throughput Screen for Inhibitors of the Prolyl
Isomerase, Pin1, Identifies a Seaweed Polyphenol that Reduces Adipose Cell
Differentiation, Bioscience, Biotechnology, and Biochemistry, 78, 832-838,
2014, http://dx.doi.org/10.1080/09168451.2014.905189.
Yuko
Cho*, Motoo Ogawa, Mari
Yotsu-Yamashita, and Yasukatsu Oshima, Effect of
5-fluoro-2f-deoxyuridine on toxin production and cell cycle regulation in
marine dinoflagellate, Alexandrium tamarense, Harmful Algae, 32, 64-72,
2014.
Atsuo Nakazaki, Yuki Ishikawa, Yusuke
Sawayama, Mari Yotsu-Yamashita,
Toshio Nishikawa*, Synthesis of crambescin B carboxylic acid, a potent
inhibitor of voltage-gated sodium channels, Org. Biomol. Chem., 12,
53-56, 2014.
Yoshihisa Tanaka, Masayuki Satake,* Mari
Yotsu-Yamashita, and Yasukatsu Oshima,
Gymnocin-A carboxylic acid and gymnocin-a2,
cytotoxic polyethers from the red tide
dinoflagellate Karenia mikimotoi, Heterocycles, 87 (10),
2037-2046, 2013.
Keiichi
Konoki*, Tatsuya Onoda,
Sachie Furumochi, Yuko
Cho, Mari Yotsu-Yamashita,
Takeshi Yasumoto, The binding of okadaic acid analogues to recombinant OABP2.1
originally isolated from the marine sponge Halichondria okadai, Bioorg.
Med. Chem. Lett., 23, 5833-5835, 2013.
Takafumi Akimoto, Asako Masuda, Mari
Yotsu-Yamashita, Takatsugu Hirokawa, and Kazuo,
Nagasawa*, Synthesis of saxitoxin derivatives bearing guanidine and urea groups
at C13 and evaluation of their inhibitory activity on voltage-gated sodium
channel. Org. Biomol. Chem., 11, 6642-6649, 2013.
@
Mari
Yotsu-Yamashita*, Yuka Abe,
Yuta Kudo, Raphael
Ritson-Williams, Valerie J. Paul, Keiichi Konoki,
Yuko Cho, Masaatsu
Adachi, Takuya Imazu, Toshio Nishikawa, and Minoru Isobe. First identification
of 5,11-dideoxytetrodotoxin in marine animals, and characterization of major
fragment ions of tetrodotoxin and its analogs by high resolution ESI-MS/MS. Marine
Drugs 11, 2799-2813, 2013. Open access: (http://www.mdpi.com/1660-3397/11/8/2799/)@ doi:10.3390/md11082799
Mari
Yotsu-Yamashita*, Natsumi Okoshi,
Kouichi Watanabe, Nao Araki,
Hiroe Yamaki, Yuki
Shoji, and Takahiro Terakawa, Localization of
pufferfish saxitoxin and tetrodotoxin binding protein (PSTBP) in the tissues of
the pufferfish, Takifugu pardalis, analyzed by immunohistochemical
staining, Toxicon, 72, 23-28, 2013.
Haruhiko Fuwa*, Masato Kawakami,
Kenkichi Noto, akashi Muto, Yuto Suga, Keiichi Konoki,
Mari Yotsu-Yamashita,
Makoto Sasaki, Concise Synthesis and Biological Assessment of (+)-Neopeltolide
and a 16-Member Stereoisomer Library of 8,9-Dehydroneopeltolide: Identification
of Pharmacophoric Elements, Chem. Eur. J., 19, 8100-8110, 2013.
Kazuya Ishigai, Haruhiko Fuwa,* Keisuke
Hashizume, Ryo Fukazawa, Yuko Cho,
Mari Yotsu-Yamashita,
and Makoto Sasaki*, Total Synthesis and Biological Evaluation of (+)-Gambieric
Acid A and Its Analogues, Chem. Eur. J., 19, 5276-5288, 2013.
Yuko
Cho*, Ryoko Ozeki, Mari
Yotsu-Yamashita, and Yasukatsu Oshima,
Single-cell analysis of paralytic shellfish toxins in Alexandrium tamarense
by HPLC with post-column fluorescent derivatization Harmful Algae. Harmful
Algae, 25, 47-53, 2013.
Keiichi
Konoki*, Tatsuya Onoda,
Ryuichi Watanabe, Yuko Cho,
Shinnosuke Kaga, Toshiyuki Suzuki, and Mari Yotsu-Yamashita,
In vitro Acylation of Okadaic Acid in the Presence of Various Bivalvesf
Extracts. Marine Drugs, 11, 300-315, 2013 (Open access: http://www.mdpi.com/1660-3397/11/2/300)
Mari
Yotsu-Yamashita*, Sawako Kondo,
Shinya Segawa , Yi-chin Lin,
Haruhiko Toyohara, Hisatomi Ito, Keiichi Konoki,
Yuko Cho and Takafumi
Uchida, Isolation and Structural Determination of Two Novel Phlorotannins from
the Brown Alga Ecklonia kurome Okamura, and Their Radical Scavenging
Activities, Marine Drugs, accepted 11, 165-183, 2013 (Open access: http://www.mdpi.com/1660-3397/11/1/165).
Noriyoshi Teramoto*, Hai-Lei Zhu, Mari
Yotsu-Yamashita, Tetsuichiro Inai, Thomas C.
Cunnane, Resurgent-like cur rents in mouse vas deferens myocytes are mediated
by NaV1.6 voltage-gated sodium channels, Pfl_gers Archiv - European Journal
of Physiology, 464, 493-502, 2012.
Dietrich Mebs*, Mari
Yotsu-Yamashita, Hanns Martin Seitz, Osamu
Arakawa, Tetrodotoxin does not protect red-spotted newts, Notophthalmus
viridescens, from intestinal parasites. Toxicon, 60, 66-69, 2012.
Yuta
Kudo, Takeshi Yasumoto, Keiichi
Konoki, Yuko Cho, Mari Yotsu-Yamashita*,
Isolation and structural determination of the first 8-epi-type
tetrodotoxin analogs from the newt, Cynops ensicauda popei, and
comparison of tetrodotoxin analogs profiles of this newt and the puffer fish, Fugu
poecilonotus, Marine Drugs, 10, 655-667, 2012. (Open access:
http://www.mdpi.com/1660-3397/10/3/655/)
Mari
Yotsu-Yamashita, John Gilhen,
Ronald W. Russell, Kenneth L. Krysko, Christian Melaun, Alexander Kurz, Silke
Kauferstein, Dusan Kordis, Dietrich Mebs*, Variability of tetrodotoxin and of
its analogues in the red-spotted newt, Notophthalmus viridescens
(Amphibia: Urodela: Salamandridae), Toxicon, 59, 257-264, 2012.
Yoshikazu
Kanai, Sadahiko Hiroki,
Hiroyuki Koshino, Keiichi Konoki, Yuko Cho,
Mirriam Cayme, Yasuo Fukuyo, and Mari Yotsu-Yamashita*, Identification
of novel oxidized levuglandin D2 in
marine red alga and mouse tissues. J. Lipid Res., 52 (12), 2245-2254,
2011.
Ryoko Shinohara, Osamu Iwamoto,
Takafumi Akimoto, Mari Yotsu-Yamashita,
Kaoru Yamaoka, and Kazuo Nagasawa*, Synthesis of skeletal analogs of saxitoxin
derivatives and evaluation of their inhibitory activity on sodium ion channels
Nav1.4
and Nav1.5.
Chemistry- A European Journal, 17 (43), 12144-12152, 2011.
Eva Cagide, M Carmen Louzao, Bego_a
Espi_a, Isabel Rodriguez Ares, Mercedes, R. Vieytes, Makoto Sasaki, Haruhiko
Fuwa, Chihiro Tsukano, Yu Konno, Mari Yotsu-Yamashita,
Leo A Paquette, Takeshi Yasumoto, and Luis M. Botana*, Chem. Res. Toxicol.,
24(6), 835-842, 2011.
Q.T.
Islam, M.A.Razzak, M.A.Islam, M.I.Bari,
A.Basher, F.R.Chowdhury, A.B.M. Sayeduzzaman, H.A.M.N.Ahasan, M.A.Faiz,
O.Arakawa, M. Yotsu-Yamashita,
U.Kuch*, D.Mebs, Puffer fish poisoning in
Bangladesh: clinical and toxicological results from large out breaks in 2008, Trans. Roy. Soc. Trop. Med. Hyg.,
105, 74-80, 2011.
Mari
Yotsu-Yamashita*, Jun-Ho Jang, Yuko
Cho, Keiichi Konoki, Optimization
for simultaneous analysis of tetrodotoxin, 4-epitetrodotoxin,
4,9-anhydrotetrodotoxin and 5,6,11-trideoxytetrodotoxin by hydrophilic
interaction liquid chromatography-tandem mass spectrometry, Forensic Toxicol.,
29, 61-64, 2011.
Keiichi
Konoki,* Kaori Saito, Hiroki Matsuura,
Naoyuki Sugiyama, Yuko Cho, Mari Yotsu-Yamashita,
and Kazuo Tachibana, Binding of diarrheic shellfish poisoning toxins to okadaic
acid binding proteins purified from the sponge Halichondria okadai, Bioorg.
Med. Chem., 18 (21), 7607-7610, 2010.
Jun-Ho Jang, Jong-Soo Lee and Mari
Yotsu-Yamashita*, LC/MS Analysis of Tetrodotoxin
and Its Deoxy Analogs in the Marine Puffer Fish Fugu niphobles from the
Southern Coast of Korea, and in the Brackishwater Puffer Fishes Tetraodon
nigroviridis and Tetraodon biocellatus from Southeast Asia, Marine Drugs, 8,
1049-1058, 2010.
Dietrich Mebs*, Osama Arakawa, Mari
Yotsu-Yamashita, Tissue distribution of
tetrodotoxin in the red-spotted newt Notophthalmus viridescens, Toxicon, 55,
1353-1357, 2010.
Mari
Yotsu-Yamashita* Hiroe Yamaki, Natsumi Okoshi
and Nao Araki, Distribution of homologous proteins to puffer fish saxitoxin and
tetrodotoxin binding protein in the plasma of puffer fish and among the tissues
of Fugu pardalis examined by Western blot analysis, Toxicon, 55, 1119-1124,
2010.
Mari
Yotsu-Yamashita* and Eiko Tateki, First report
on toxins in the Panamanian toads Atelopus limosus, A. glyphus and A. certus,
Toxicon, 55, 153-156, 2010.
Haruhiko Fuwa*, Asami Saito, Shinya
Naito, Keiichi Konoki, Mari Yotsu-Yamashita,
and Makoto Sasaki, Total Synthesis and Biological Evaluation of
(+)-Neopeltolide and Its Analogues, Chem. Eur. J. 15, 12807-12818, 2009.
Mari
Yotsu-Yamashita*, Analytical methods for
tetrodotoxin and its analogs. Journal of medical technology, 53 (6),
707-711, 2009. (in Japanese)
Jun-Ho Jang and Mari
Yotsu-Yamashita*, Tetrodotoxin and
5,6,11-trideoxytetrodotoxin in Tetraodon nigroviridis and T. biocellatus
collected from Southeast Asia, The proceedings of the 5th World Fisheries
Congress (WFC), 2008.10.20-24 Yokohama. (Proceeding) 2008.
M. Carmen Louzao, Eva Cagide, Mari
Yotsu-Yamashita, and Makoto Sasaki, Polycavernosides and Gambierol: Chemistry,
Pharmacology, Toxicology, and Detection, in Seafood and Freshwater Toxins:
Pharmacology, Physiology and detection (Food Science and Technology), Part
VIIIE, Chaptor28, 597-628 (total 960 pages), Editor, Louis M. Botana, Crc Pr I
Llc (Review), 2008.
Michiko Kono, Takashi Matsui, Kiyoshi
Furukawa, Takuhiko Takase, Kunio Yamamori, Hideko Kaneda, Daisuke Aoki, Jun-Ho
Jang, Mari Yotsu-Yamashita*, Examination of transformation among tetrodotoxin
and its analogs in the living cultured juvenile puffer fish, kusafugu, Fugu
niphobles by intramuscular administration, Toxicon, 52, 714-720, 2008.
Yuko Cho*, Noriyuki Ogawa, Miyako
Takahashi, His-Pin Lin, Yasukatsu Oshima, Purification and characterization of
paralytic shellfish toxin transforming enzyme, sulfocarbamoylase I, from
Japanese bivalve, Peronidia venulosa. Biochimica et Biophysica Acta - Proteins
and Proteomics, 1784 (2008), pp. 1277-1285.
Yuko Cho*, Kaori Hiramatsu, Motoo
Ogawa, Takuo Omura, Takashi Ishimaru and Yasukatsu Oshima, Non-toxic and toxic
subclones obtained from a toxic clonal culture of Alexandrium tamarense
(Dinophyceae): Toxicity and molecular biological feature, Harmful Algae, 7
(2008), pp. 740-751.
Michiko Kono*, Takashi Matsui, Kiyoshi
Furukawa, Mari Yotsu-Yamashita, Kunio Yamamori, Accumulation of tetrodotoxin
and@4,9-anhydrotetorodotoxin in cultured juvenile kusafugu Fugu niphobles by
dietary administration of natural toxic komonfugu Fugu poecilonotus liver.
Toxicon, 41, 1269-1273, 2008.
Satoshi Maruta, Kaoru Yamaoka and Mari
Yotsu-Yamashita*, Two critical residues in p-loop regions of puffer fish Na+
channels on TTX-sensitivity, Toxicon, 51, 381-387, 2008.
Jun-Ho Jang and Mari Yotsu-Yamashita*,
6,11-Dideoxytetrodotoxin from the puffer fish, Fugu pardalis. Toxicon, 50,
947-851, 2007.
Leo A. Paquette and Mari
Yotsu-Yamashita, Polycavernosides, In "Phycotoxins: chemistry and
biochemistry", Captor 15, P275-296, 2007, Ames, Iowa, USA, Blackwell Publ.
(Review)
Mari Yotsu-Yamashita, Dietrich Mebs*,
Axel Kwet and Michael Schneider, Tetrodotoxin and its analogue
6-epitetrodotoxin in newts (Triturus spp.; Urodela, Salamandridae) from
southern Germany, Toxicon, 50, 306-309, 2007.
Mari Yotsu-Yamashita*, Kazumi Abe,
Tetsuya Seki, Kenshu Fujiwara, and Takeshi Yasumoto, Polycavernoside C and C2,
the new analogs of the human lethal toxin polycavernoside A, from the red alga,
Gracilaria edulis, Tetrahedron Lett. 48, 2255-2259 (2007)
Mari Yotsu-Yamashita, Dietrich Mebs*
and Wolfgang Flachsenberger, Distribution of tetrodotoxin in the body of the
blue-ringed octopus (Hapalochlaena maculosa) Toxicon, 49, 410-412 (2007).
E. Cagide1, M.C. Louzao1, I.R. Ares1,
M.R. Vieytes2, M. Yotsu-Yamashita3, L.A. Paquette4, T. Yasumoto5 and L.M.
Botana1,* EFFECTS OF A SYNTHETIC ANALOG OF POLYCAVERNOSIDE A ON HUMAN
NEUROBLASTOMA CELLS, Cell Physiol. Biochem., 19, 185-194 (2007).
Mitsugu Akagawa, Yoshihisa Ishii,
Takeshi Ishii, Takashiro Shibata, Mari Yotsu-Yamashita, Kyozo Suyama, and Koji
Uchida*, Metal-catalyzed oxidation of protein-bound dopamine, Biochemistry,
2006, 45, 15120-15128.
Tomoko Aoki, Daisuke Sasaki, Mitsugu
Akagawa, Kyozo Suyama, and Mari Yotsu-Yamashita*, Application of Lysyl Oxidase
Activity in Egg Shell Membrane of Hen to Selective Oxidation of e-Amino Group
in Lysine to Aldehyde in Short Peptides, Peptide Science 2006, 126. H. Ishida
and H. Mihara (Ed.) The Japanese peptide society
Akagawa, M*., Sasaki, D., Ishii, Y.,
Kurota, Y., Yotsu-Yamashita, M., Uchida, K., and Suyama, K. Chem. Res.
Toxicol.19, 1059-1065 (2006).
Junho Jang and Mari Yotsu-Yamashita*,
Distribution of tetrodotoxin, saxitoxin, and their analogs among tissues of the
puffer fish Fugu pardalis. Toxicon 48, 980-987 (2006).
Louzao, M. C., Vieytes, M. R.,
Yasumoto, T., Yotsu-Yamashita, M., and Botana, L. M.,* Changes in membrane
potential: An early signal triggered by neurologically active phycotoxins.
Chem. Res. Toxicol., 19, 788-793 (2006) .
MariYotsu-Yamashita, Spectroscopic
study of the structure of zetekitoxin AB, Marine Natural Products Series:
Topics in Heterocyclic Chemistry, Vol. 5, 53-65 Kiyota, Hiromasa (Ed.),
Publisher: Springer-Verlag GmbH (2006).
Toshio Nakagawa, Junho Jang, and Mari
Yotsu-Yamashita*, Hydrophilic interaction liquid chromatography-electrospray
ionization mass spectrometry of tetrodotoxin and its analogs, Anal. Biochem.,
352, 142-144 (2006).
Raphael
Ritson-Williams*, Mari Yotsu-Yamashita, and Valerie Paul, Ecological functions
of tetrodotoxin in a deadly polyclad flatworm, Proc. Natl. Acad. Sci. USA, 103
(9) 3176-3179 (2006).
Mari Yotsu-Yamashita* and Daisuke
Sasaki, Identification of the cyclic aminal forms of a-aminoadipic and
g-glutamic semialdehydes as the major forms in aqueous solution, Peptide
Science 2004: Y. Shimohigashi (Ed.) The Japanese peptide society 435-436
(2005).
Mari Yotsu-Yamashita*, Ayako Goto, and
Toshio Nakagawa, Isolation of 4-S-cysteinyltetrodotoxin from the liver of the
puffer fish Fugu pardalis, and formation of the adducts of
4,9-anhydrotetrodotoxin with thiols, Chem. Res. Toxicol. 18, 865-871(2005).
Mari
Yotsu-Yamashita*, Takeshi Yasumoto, Shunichi Yamada , Fe Farida A.
Bajarias, Mirriam A. Formelozsa|, Marc Lawrence Romero, and Yasuo Fukuyo||
Identification of polycavernosides A as the causative agent of the fatal food
poisoning resulting from ingestion of the red alga Gracilaria edulis in
Philippines, Chem. Res. Toxicol. 17, 1265-1271 (2004).
Mari Yotsu-Yamashita,
"Possible mechanism for resistibility of puffer fish against
tetrodotoxin", Jpn. J. Physiol., 54, Suppl., S13 (2004).
Mari Yotsu-Yamashita*, Yong H. Kim*,
Samuel C. Dudley Jr., Gaurav Choudhary, Arnold Pfahnl, Yasukatsu Oshima and
John W. Daly, Ҽ/span>The
structure of zetekitoxin AB, a saxitoxin analog from the golden frog Atelopus
zeteki:: a potent sodium channel blockerӼ/span>. Proc. Natl. Acad. Sci. USA,
101 (13), 4346-4351 (2004).
Yong Hae Kim
and Mari Yotsu-Yamashita, Ҽ/span>Potent
neurotoxins: Tetrodotoxin, chiriquitoxin, and zetekitoxin from Atelopus frogs
of Central AmericaӼ/span>, J. Toxicol. Toxin Reviews, 22 (4), 521-532, 2003.
Mari Yotsu-Yamashita and Dietrich
Mebs*, Ҽ/span>Occurrence
of 11-oxotetrodotoxin in the red-spotted newt, Notophthalmus viridescens, and
further studies on the levels of tetrodotoxin and its analogues in the newt
eftsӼ/span>, Toxicon, 41, 893-897, 2003.
Mari Yotsu-Yamashita*, Daisuke Urabe,
Masanori Asai, Toshio Nishikawa and Minoru Isobe, Ҽ/span>Biological
activity of 8,11-dideoxytetrodotoxin: Lethality to mice and the inhibitory
activity to cytotoxicity of ouabain and veratridine in mouse neuroblastoma
cells, Neuro-2aӼ/span>, Toxicon, 42, 557-560, 2003.
Gaurav Choudhary, Mari Yotsu-Yamashita,
Lisa Shang, Takeshi Yasumoto and Samuel C. Dudley, Jr.*, Ҽ/span>Interactions
of C-11 Hydroxyl of Tetrodotoxin with the Sodium Channel Outer
VestibuleӼ/span>, Biophys. J., 84, 287-294, 2003.
Misako Tagiri-Endo, Kaori Ono, Kiyotaka
Nakagawa, Mari Yotsu-Yamashita and Teruo Miyazawa,* Ҽ/span>Ozonation
of phosphatidylcholine in ethanol: separation and identification of a novel
ethoxyhydroperoxideӼ/span>, Lipids, 37 (10), 1007-1012, 2002.
Mari Yotsu-Yamashita, Sodium Channels
of Puffer Fish, Nippon Suisan Gakkaishi, 68 (6), 925-926, 2002.
Mari Yotsu-Yamashita, Yuki Shoji,
Takahiro Terakawa, Shunichi Yamada, Teruo Miyazawa and Takeshi Yasumoto, Ҽ/span>Mutual
inhibition of tetrodotoxin and saxitoxin binding to their binding protein from
plasma of the puffer fish, Fugu pardalisӼ/span>, Biosci. Biotechnol.
Biochem., 66 (11), 2520-2524, 2002.
Mari Yotsu-Yamashita, Atsuko Sugimoto,
Takahiro Terakawa, Yuki Shoji, Teruo Miyazawa and Takeshi Yasumoto, Ҽ/span>Purification,
chracterization, and cDNA cloning of a novel soluble saxitoxin and tetrodotoxin
binding protein from plasma of the puffer fish, Fugu pardalisӼ/span>, Eur.
J. Biochem, 268 (22), 5937-5946, 2001.
Mari Yotsu-Yamashita, Ҽ/span>Chemistry
of puffer fish toxinӼ/span>, J. Toxicol. Toxin Reviews, 20 (1), 51-66, 2001.
Mari Yotsu-Yamashita and Dietrich Mebs,
Ҽ/span>The
levels of tetrodotoxin and its analogue 6-epitetrodotoxin in the red-spotted
newts, Notophthalmus viridescens, Toxicon, 39, 1261-1263, 2001.
Yuki Shoji, Mari Yotsu-Yamashita, Teruo
Miyazawa and Takeshi Yasumoto, Ҽ/span>Electrospray
Ionization Mass Spectrometry of Tetrodotoxin and its Analogs: Liquid
Chromatography/ Mass Spectrometry, Tandem Mass Spectrometry, and Liquid
Chromatography/Tandem Mass SpectrometryӼ/span>, Anal. Biochem., 290 (1),
10-17, 2001.
Mari Yotsu-Yamashita, Katsuhiko
Nishimori, Yoko Nitanai, Masako Isemura, Atsuko Sugimoto and Takeshi Yasumoto,
Ҽ/span>Binding Properties of 3H-PbTx-3 and 3H-saxitoxin to Brain Membranes
and to Skeletal Muscle Membranes of Puffer Fish Fugu pardalis, and the Primary
Structure of a Voltage-Gated Na+ Channel a-Subunit (fMNa1) from Skeletal Muscle
of F. pardalisӼ/span>, Biochem. Biophys. Res. Commun., 267 (1), 403-412,
2000.
E. J. Nunez-Vazquez, M.
Yotsu-Yamashita, A. P. Sierra-Beltran, T. Yasumoto and J. L. Ochoa,
"Toxicities and distribution of tetrodotoxin in the tissues of puffer fish
found in the coast of the Baja California Peninsula, Mexico", Toxicon, 38,
729-734, 2000.
Charles T. Hanifin, Mari
Yotsu-Yamashita, Takeshi Yasumoto, Edmund D. Broodie III and Edmund D. Broodie
Jr., "Toxicity of dangerous prey:variation of tetrodotoxin levels within
and among populations of the newt Taricha granulosa ", J. Chem. Ecology,
25, 2161-2175, 1999.
Louis Barriault, Serge L. Boulet,
Kenshu Fujiwara, Akio Murai, Leo A. Paquette and Mari Yotsu-Yamashita,
"Synthesis and biological evaluation of analogs of the marine toxin
polycavernoside A", Bioorg.& Med.Chem. Lett., 9, 2069-2072, 1999.
Mari Yotsu-Yamashita, Atsuko Sugimoto,
Akira Takai and Takeshi Yasumoto, "Effects of Specific Modifications of
Several Hydroxyls of Tetrodotoxin on its Affinity to Rat Brain Membrane",
J. Pharmacol. Exp. Ther., 289 (3), 1688-1696, 1999.
Mari
Yotsu-Yamashita, Bernhard Schimmele and Takeshi Yasumoto, "Isolation and
Structural Assignment of 5-Deoxytetrodotoxin from the Puffer Fish, Fugu
poecilonotus ", Biosci. Biotechnol. Biochem., 63, 961-963, 1999.