２０２１年度

Lactobacillus delbrueckii CRL 581 differentially modulates 2 TLR3-triggered antiviral innate immune response in intestinal 3 epithelial cells and macrophages Mariano Elean†, Leonardo Albarracín†, Kohtaro Fukuyama†, Binghui Zhou, Mikado Tomokiyo, Shugo Kitahara, Shota Araki, Yoshihito Suda, Lucila Saavedra, Julio Villena*, Elvira M. Hebert*, Haruki Kitazawa* (†:Equal contribution) Microorganisms, 9:2449 1-18, Dec, 2021

Novel LysM motifs for antigen display on lactobacilli for mucosal immunization Fernanda Raya-Tonetti, Melisa Müller, Jacinto Sacur, Haruki Kitazawa*, Julio Villena*†, Maria Guadalupe Vizoso-Pinto*†(†: Equal contribution) Sci Rep, 11:21691 1-11, Nov 4, 2021

NOD2 genotypes affect the symptoms and mortality in the porcine circovirus 2-spreading pig population Kasumi Suzuki, Hiroki Shinkai, Gou Yoshioka, Toshimi Matsumoto, Junji Tanaka, Noboru Hayashi, Haruki Kitazawa*, Hirohide Uenishi* Genes, 12:1424 1-11, Sep 16, 2021

Immunobiotic Lactobacilli Improve Resistance of Respiratory Epithelial Cells to SARS-CoV-2 Infection Md. Aminul Islam, Leonardo Albarracin, Mikado Tomokiyo, Juan Carlos Valdez, Jacinto Sacur, Maria Guadalupe Vizoso-Pinto, Bruno G. N. Andrade, Rafael R. C. Cuadrat, Haruki Kitazawa, Julio Villena Pathogens, 10:1197 1-16, Sep 15, 2021

The gut microbiota induces Peyer’s-patch-dependent secretion of maternal IgA into milk Katsuki Usami, Kanae Niimi, Ayumi Matsuo, Yoshihisa Suyama, Yoshifumi Sakai, Shintaro Sato, Kohtaro Fujihashi, Hiroshi Kiyono, Saeka Uchino, Mutsumi Furukawa, Jahidul Islam, Kaori Ito, Taiki Moriya, Yutaka Kusumoto, Michio Tomura, Russell C. Hovey, Junichi Sugawara, Hiroshi Yoneyama, Haruki Kitazawa, Kouichi Watanabe, Hisashi Aso, Tomonori Nochi Cell Reports, 36(10):109655 1-39, Sep 7, 2021

Genome-wide association studies for production, respiratory disease, and immune-related traits in Landrace pigs Yoshinobu Uemoto, Kasumi Ichinoseki, Toshimi Matsumoto, Nozomi Oka, Hironori Takamori, Hiroshi Kadowaki, Chihiro Kojima-Shibata, Eisaku Suzuki, Toshihiro Okamura, Hisashi Aso, Haruki Kitazawa, Masahiro Satoh, Hirohide Uenishi, Keiichi Suzuki Sci Rep, 11:15823 1-11, Aug 4, 2021

Evaluation of Porcine Intestinal Epitheliocytes as an In vitro Immunoassay System for the Selection of Probiotic Bifidobacteria to Alleviate Inflammatory Bowel Disease Nana Sato†, Mao Yuzawa†, Md Aminul Islam, Mikado Tomokiyo, Leonardo Albarracin, Valeria Garcia-Castillo, Wakako Ideka-Ohtsubo, Noriyuki Iwabuchi, Jin-zhong Xiao, Apolinaria Garcia-Cancino, Julio Villena*, Haruki Kitazawa* (†: Equal contribution) Probiotics Antimicrob Proteins, 13(3) 824-836, Jun, 2021

Dolosigranulum pigrum Modulates Immunity against 2 SARS-CoV-2 in Respiratory Epithelial Cells Md. Aminul Islam, Leonardo Albarracin, Vyacheslav Melnikov, Bruno G. N. Andrade, Rafael R.C. Cuadrat, Haruki Kitazawa*, Julio Villena* Pathogens, 10:634 1-11, May 21, 2021

Ligilactobacillus salivarius Strains Isolated From the Porcine Gut Modulate Innate Immune Responses in Epithelial Cells and Improve Protection Against Intestinal Viral-Bacterial Superinfection. Yuhki Indo, Shugo Kitahara, Mikado Tomokiyo, Shota Araki, Md Aminul Islam, Binghui Zhou, Leonardo Albarracin, Ayako Miyazaki, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Takato Takenouchi, Hirohide Uenishi, Hisashi Aso, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Haruki Kitazawa Frontiers in immunology, 12:652923 1-22, May, 2021

Immunobiotic Feed Developed with Lactobacillus delbrueckii subsp. delbrueckii TUA4408L and the Soymilk By-Product Okara Improves Health and Growth Performance in Pigs Yoshihito Suda, Nana Sasaki, Kyoma Kagawa, Mariano Elean, Binghui Zhou, Mikado Tomokiyo, Md Aminul Islam, Muhammad Shahid Riaz Rajoka, A K M Humayun Kober, Tomoyuki Shimazu, Shintaro Egusa, Yuji Terashima, Hisashi Aso, Wakako Ikeda-Ohtsubo, Julio Villena, Haruki Kitazawa Microorganisms, 9(921) 1-18, Apr 25, 2021

Lactiplantibacillus plantarum as a Potential Adjuvant and Delivery System for the Development of SARS-CoV-2 Oral Vaccines Julio Villena†, Chang Li†, Maria Guadalupe Vizoso-Pinto, Jacinto Sacur, Linzhu Ren*, Haruki Kitazawa* (†:Equal contribution) Microorganisms, 9:683(4) 1-26, Mar 26, 2021

Effect of Dietary Supplementation of Immunobiotic Lactiplantibacillus plantarum N14 Fermented Rakkyo (Allium chinense) Pickled Juice on the Immunocompetence and Production Performance of Pigs Md. Aminul Islam†, Kenji Hashiguchi†, AKM Humayun Kober, Kyoko Morie, Binghui Zhou, Mikado Tomokiyo, Tomoyuki Shimazu, Hisashi Aso, Julio Villena*, Yoshihito Suda*, Haruki Kitazawa* (†:Equal contribution) Animals, 11:752 1-18, Mar, 2021

Characterization of sugar nucleotides in colostrum of dairy domestic farms animals Yuri Mineguchi, Kyosuke Goto, Yuna Sudo, Kentaro Hirayama, Hirotoshi Kashiwagi, Izumi Sasagase, Haruki Kitazawa, Sadaki Asakuma, Kenji Fukuda, Tadasu Urashima* Int Dairy J, 113:104897 1-14, Feb, 2021

Improvement of disease resistance in livestock: application of immunogenomics and CRISPR/Cas9 technology Md. Aminul Islam, Sharmin Aqter Rony, Mohammad Bozlur, Rahman, Mehmet, Ulas Cinar, Julio Villena, Muhammad Jasim Uddin*, Haruki Kitazawa* Animals, 10(2236) 1-20, Nov 28, 2020

２０２０年度

Functional and Genomic Characterization of Ligilactobacillus salivarius TUCO-L2 Isolated From Lama glama Milk: A Promising Immunobiotic Strain to Combat Infections Sandra Quilodrán-Vega, Leonardo Albarracin, Flavia Mansilla, Lorena Arce, Binghui Zhou, Md Aminul Islam, Mikado Tomokiyo, Imad Al Kassaa, Yoshihito Suda, Haruki Kitazawa, Julio Villena Frontiers in Microbiology, 11:608752 1-24, Nov, 2020

Bayesian latent class evaluation of three tests for the screening of subclinical caprine mastitis in Bangladesh Md. Aminul Islam, Sharmin Aqter Rony, Haruki Kitazawa*, AKM Anisur Rahman Tropical Animal Health and Production, 52(6) 2873-2881, Nov, 2020

Selection of immunobiotic Ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pig: functional and genomic studies Zhou, B†, L. Albarracin†, Y. Indo, L. Arce, Y. Masumizu, M. Tomokiyo, Md. A. Islam, V. Garcia-Castillo, W. Ikeda-Ohtsubo, T. Nochi, H. Morita, H. Takahashi, S. Kurata, J. Villena*, H. Kitazawa*, (†:Equal contribution) Microorganisms, 8(1659) 1-30, Oct, 2020

Editorial: Nutrition, Immunity and Viral Infections Julio Villena*, Takeshi Shimosato, Maria G. Vizoso Pinto, Haruki Kitazawa* 7:125 1-2, Sep 8, 2020

IL-12p40 gene expression in lung and hilar lymph nodes of MPS resistant pigs Akiko Sakuma, Shizuka Sugawara, Hikaru Hidaka, Mitsuru Nakajo, Yoshihito Suda, Tomoyuki Shimazu, Michael T. Rose, Megumi Urakawa, Tao Zhuang, Guoqi Zhao, Kouichi Watanabe, Tomonori Nochi, Haruki Kitazawa, Kazuo Katoh, Keiichi Suzuki, Hisashi Aso Animal Science Journal, 91(1) e13450, Sep 3, 2020

Alveolar macrophages are key players in the modulation of the respiratory antiviral immunity induced by orally administered Lacticaseibacillus rhamnosus CRL1505 Valeria García-Castillo†, Mikado Tomokiyo†, María Fernanda, Raya Tonetti, Md. Aminul Islam, Hideki Takahashi, Haruki Kitazawa*, Julio Villena* (†: Equal contribution) Front. Immunol., 11(568636) 1-20, Sep, 202

Draft Genome Sequence of Ligilactobacillus salivarius FFIG58 Isolated from the Intestinal Tract of Wakame-fed Pig Binghui Zhou, Leonardo Albarracin, Yuki Masumizu, Yuhki Indo, Md. Aminul Islam, Valeria Garcia-Castillo, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Julio Villena, Haruki Kitazawa Microbiology Resource Announcements, 9(34) e00839-20, Aug 20, 202

Immunobiotic Lactobacillus jensenii TL2937 Alleviates Dextran Sodium Sulfate-Induced Colitis by Differentially Modulating the Transcriptomic Response of Intestinal Epithelial Cells. Nana Sato, Valeria Garcia-Castillo, Mao Yuzawa, Md Aminul Islam, Leonardo Albarracin, Mikado Tomokiyo, Wakako Ikeda-Ohtsubo, Apolinaria Garcia-Cancino, Hideki Takahashi, Julio Villena, Haruki Kitazawa Frontiers in immunology, 11(2174) 1-21, Aug, 202

Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics. Asuka Tada, Akm Humayun Kober, Md Aminul Islam, Manami Igata, Michihiro Takagi, Masahiko Suzuki, Hisashi Aso, Wakako Ikeda-Ohtsubo, Kazutoyo Yoda, Kenji Miyazawa, Fang He, Hideki Takahashi, Julio Villena, Haruki Kitazawa Cells, 9:1715(7) 1-21, Jul 17, 202

Factors affecting decreasing viscosity of the culture medium during the stationary growth phase of exopolysaccharide-producing Lactobacillus fermentum MTCC 25067 Bharat Mengi, Shinya Ikeda, Daiki Murayama, Hiroki Bochimoto, Shinpei Matsumoto, Haruki Kitazawa, Tadasu Urashima, Kenji Fukuda Bioscience of microbiota, food and health, 39(3) 160-168, Jul, 2020

The role of alveolar macrophages in the improved protection against Respiratory Syncytial Virus and pneumococcal superinfection induced by the peptidoglycan of Lactobacillus rhamnosus CRL1505 Patricia Clua, Mikado Tomokiyo, Fernanda Raya Tonetti, Md. Aminul Islam, Valeria García Castillo, Guillermo Marcial, Susana Salva, Susana Alvarez, Hideki Takahashi, Shoichiro Kurata, Haruki Kitazawa, Julio Villena Cells, 9:1653(7) 1-27, Jul, 2020

Intestinal microbiota and immune modulation in zebrafish by fucoidan from Okinawa mozuku (Cladosiphon okamuranus) Wakako Ikeda-Ohtsubo*, Adrià López Nadal, Edoardo Zaccaria, Masahiko Iha, Haruki Kitazawa, M Kleerebezem, Sylvia Brugman* 7:67 1-12, Jun 24, 2020

The Modulation of Mucosal Antiviral Immunity by Immunobiotics: Could They Offer Any Benefit in the SARS-CoV-2 Pandemic? Julio Villena, Haruki Kitazawa Frontiers in Physiology, 11(699) 1-20, Jun 16, 2020

Evaluation of the Immunomodulatory Ability of Lactic Acid Bacteria Isolated from Feedlot Cattle Against Mastitis Using a Bovine Mammary Epithelial Cells In Vitro Assay Kohtaro Fukuyama, Md. Aminul Islam, Michihiro Takagi, Wakako Ikeda-Ohtsubo, Shoichiro Kurata, Hisashi Aso, Graciela Vignolo, Julio Villena, Haruki Kitazawa Pathogens, 9:410(5) 1-15, Jun, 2020

Exopolysaccharides from Streptococcus thermophilus ST538 modulate the antiviral innate immune response in porcine intestinal epitheliocytes Hiroya Mizuno† , Kae Tomotsune† , Md. Aminul Islam, Ryutaro Funabashi, Leonardo Albarracin, Wakako Ikeda-Ohtsubo, Hisashi Aso, Hideki Takahashi, Katsunori Kimura, Julio Villena, Yasuko Sasaki*, Haruki Kitazawa*, (†: Equal contribution) Front. Microbiol., 11:894 1-14, May 19, 2020

The Ability of Respiratory Commensal Bacteria to Beneficially Modulate the Lung Innate Immune Response Is a Strain Dependent Characteristic Moyano, R. O. F. R. Tonetti†, M. Tomokiyo†, P. Kanmani, M. G. Vizoso-Pinto, H. Kim, S. Quilodrán-Vega, V. Melnikov, S. Alvarez, H. Takahashi, S. Kurata, H. Kitazawa*, Julio Villena* (†: Equal contribution) Microorganisms, 8(5):727(5) 1-23, May 13, 2020

Modulation of Toll-like receptor-mediated innate immunity in bovine intestinal epithelial cells by lactic acid bacteria isolated from feedlot cattle Flavia Mansilla, Michihiro Takagi, Valeria Garcia-Castillo, Hisashi Aso, Maria Elena Nader-Macias, Graciela Vignolo, Haruki Kitazawa, Julio Villena Benef. Microbes, 11(3) 269-282, May 4, 2020

Lipoteichoic acid is involved in the ability of the immunobiotic strain Lactobacillus plantarum CRL1506 to modulate the intestinal antiviral innate immunity triggered by TLR3 activation Hiroya Mizuno†, Lorena Arce†, Kae Tomotsune, Leonardo Albarracín, Ryutaro Funabashi, Daniela Vera, Md. Aminul Islam, Maria Guadalupe Vizoso-Pinto, Hideki Takahashi, Yasuko Sasaki, Haruki Kitazawa*, Julio Villena*, (†: Equal contribution) Front. Immunol., 11:571 1-14, Apr, 202

The exopolysaccharide of Lactobacillus fermentum UCO-979C is partially involved in its immunomodulatory effect and its ability to improve the resistance against Helicobacter pylori infection Valeria Garcia-Castillo†, Guillermo Marcial†, Leonardo Albarracín, Mikado Tomokiyo, Patricia Clua, Hideki Takahashi, Haruki Kitazawa*, Apolinaria Garcia-Cancino*, Julio Villena*, (†: Equal contribution) Microorganisms, 8(479) 1-20, Mar 27, 2020

Transcriptome Analysis of The Inflammatory Responses of Bovine Mammary Epithelial Cells: Exploring Immunomodulatory Target Genes for Bovine Mastitis. Md Aminul Islam, Michihiro Takagi, Kohtaro Fukuyama, Ryoya Komatsu, Leonardo Albarracin, Tomonori Nochi, Yoshihito Suda, Wakako Ikeda-Ohtsubo, Victor Rutten, Willem van Eden, Julio Villena, Hisashi Aso, Haruki Kitazawa Pathogens (Basel, Switzerland), 9(3):200(3) 1-18, Mar 9, 2020

Efficient selection of new immunobiotic strains with antiviral effects in local and distal mucosal sites by using porcine intestinal epitheliocytes Leonardo Albarracin†, Valeria Garcia-Castillo†, Yuki Masumizu, Yuhki Indo, Md Aminul Islam, Yoshihito Suda, Apolinaria Garcia, Hisashi Aso, Hideki Takahashi, Haruki Kitazawa*, Julio Villena*, (†: Equal contribution) Front. Immunol., 11:543 1-16, Mar, 2020

Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin. Asuka Tada, Md Aminul Islam, Akm Humayun Kober, Kohtaro Fukuyama, Michihiro Takagi, Manami Igata, Leonardo Albarracin, Wakako Ikeda-Ohtsubo, Kenji Miyazawa, Kazutoyo Yoda, Fang He, Hideki Takahashi, Julio Villena, Hisashi Aso, Haruki Kitazawa International journal of molecular sciences, 21(2) 1-20, Jan 18, 2020

Immunomodulatory properties of bacterium-like particles obtained from immunobiotic lactobacilli: prospects for their use as mucosal adjuvants Raya, F, L. Arce, S. Salva, S. Alvarez, H. Takahashi, H Kitazawa, J. Villena, M. G. Vizoso-Pinto Front. Immunol., 11:15 1-13, Jan, 2020